Solution reagent kit CHE-10 series

for clinical chemistryfor cholinesterase

For the in vitro quantitative determination of Cholinesterase in serum or plasma. METHODOLOGY Kinetic photometric test,optimized method according to the recommendation of the German Society of Clinical Chemistry (DGKC). Principle: Cholinesterase hydrolyses butyrylthiocholine under release of butyric acid and thiocholine. Thiocholine reduces yellow potassium hexacyanoferrate (III) to colorless potassium hexacyanoferrate (II).The decrease of absorbance is measured at 405 nm. INTERFERENCES Bilirubin: No interference up to 40 mg/dL. Hemoglobin: No interference up to 500 mg/dL. Lipemia: No interference in the presence of triglycerides up to 1500 mg/dL. Ascorbic acid: No interference up to 25 mg/dL. ADDITIONAL EQUIPMENT REQUIRED BUT NOT PROVIDED 1. A clinical chemistry analyzer capable maintaining constant temperature 37°C, and measuring absorbance at 405 nm. 2. Deionized water and related equipment, e.g.: pipettes 3. Analyzer specific consumables, e.g.: sample cups 4. Control and calibrator materials. 5. NaCl ( 9 g/L) solution