Enzyme reagent kit CK-101 series

for clinical chemistrycreatine kinasecopper

For the in vitro quantitative determination of Creatine Kinase (CK) activity in human serum. METHODOLOGY In the presence of creatine phosphate, CK enzyme catalyzes the reversible phosphorylation of ADP, to form ATP and creatine. The enzyme hexokinase (HK) then catalyzes the phosphorylation of glucose by the ATP formed, to produce ADP and glucose-6-phosphate (G-6-P). The G-6-P is then oxidized to 6-phosphogluconate, with the simultaneous production of NADH. Precautions: 1. This reagent is for in vitro diagnostic use only. 2. Reagent may be irritating to skin. Flush skin with water if contacted. 3. This reagent contains sodium azide as apreservative.Do not ingest.In a dry state. may react with lead and copper plumbing to form highly explosive metal azides.Upon disposal , flush with a large volume of water to prevent azide build up. Reagent 1: H360d May damage fertility or the unborn child P308+313 If exposed or concerned:get medical advice /attention Wear suitable protective equipment REAGENT PREPARATION Reagents are supplied in a two vial, ready to use, liquid form. For some analyzers, the reagents can be combined to make a working solution by mixing 4 parts of Reagent 1 with 1 part of Reagent 2 (e.g., 20mL Rgt 1 to 5mL Rgt 2). REAGENT DETERIORATION THE REAGENT SHOULD NOT BE USED IF: 1. The reagent has an absorbance greater than 0.5 at 340 nm against water. 2. The reagent fails to meet stated parameters of performance. INTERFERENCES Studies to determine the level of interference for hemoglobin, bilirubin, and lipemia were carried out. The following results were obtained: