Copper reagent kit COP-20 series

solutionfor clinical chemistrydiagnostic

For the quantitative determination of copper in serum or plasma. For in vitro diagnostic use only. METHODOLOGY Serum copper is routinely determined by atomic absorption spectrophotometry (AAS) or spectrophotometry in clinical laboratories. AAS is the preferred method but these methods generally involve the reaction of a protein-free filtrate with organic solvents or reagents for deproteinizing, and none is satisfactory in terms of sensitivity, rapidity, and easy of measurement .In this method compounds are water soluble, and react easily and quickly with the metals forming intensely colored complexes. Principle: In an acid solution (pH 4.7),copper is released from The Ceruloplasmin protein and reduced. Afterwards the cuprous ion reacts with the 3,5DiBr-PAESA.[4-(3,5-dibromo-2-pyridylazo)-N-ethyl-N-(3-sulfopropyl) aniline] stain and forms a stable coloured complex which is photometrically measured at 580 nm. The colour intensity of this complex is proportional to the amount of copper in the sample. REAGENT COMPOSITION Acetate buffer pH 4,7 ≤ 0,022 mM 3,5-DiBr-PAESA 0,2 mM Surfactant ≤ % 1 INTERFERENCES No interferences were observed to bilirubin up to 15 mg/dL , hemoglobin up to 0,5 mg/dL and triglycerides up to 1000 mg/dL. MATERIALS REQUIRED BUT NOT PROVIDED 1. Clinical chemistry analyzer or colorimeter capable maintaining constant tempereture (37°C) and measuring absorbans at 580 nm 2. Deionize water and related equipment e.g. pipettes 3. Analyzer specific consumables, e.g: sample and read cups 4. Control and calibrator materials PROCEDURE Wavelength : 580 nm Working temperature : 37°C Optical path : 1 cm Assay type : Endpoint