C-reactive protein reagent kit CRP-20 series

solutionfor clinical chemistryHIV

Quantitative determination of C-Reactive Protein (CRP) Principle: C-reactive protein (CRP) is an acute-globulin synthesized by hepatocytes. The level of CRP can rise up to more than 20 times in association with acute inflammation. High CRP levels can be detected within 24 hours of the onset of the acute phase and the peak serum concentration is achieved within 48 hours. After an inflammatory stimulus, the release of cytokines induces a variety of genes to help control infection. One class of such genes is that of carbohydrate-binding proteins to which pertains CRP. In the present of calcium ions, the protein binds carbohydrates present on the membrane of many bacteria. The detection of CRP is useful in detecting inflammation and in monitoring its progress or response to treatment. C-reactive protein (CRP) forms with the specific antibody an immunological complex. The increase of turbidity after the addition of antiserum measured at λ=340 nm is proportional to CRP concentration REAGENTS Buffered pH 7.8 Polyclonal goat anti-h CRP antibody variable Sodium azide 0.095 % PEG 3 % PRECAUTIONS Components from human origin have been tested and found to be negative for the presence of HBsAg, HCV, and antibody to HIV (1/2). However handle cautiously as potentially infectious. CALIBRATION bt products recommends using CRP calibrator . Recalibrate when control results are out of specified tolerances, when using different lot of reagent and when the instrument is adjusted. REAGENT PREPARATION Reagents are supplied in a two vial, ready to use,