HDL cholesterol reagent kit HDL-50 series

solutionfor clinical chemistrydiagnostic

For the quantitative determination high-density lipoprotein cholesterol in human serum or plasma. For in vitro diagnostic use only. Principle: The method is the direct determination of serum HDL cholesterol without pretreatment or centrifugation of the sample. The method depends only on the properties of a detergent that dissolves HDL Cholesterol, whereby HDL-cholesterol is released to react with cholesterol esterase. Cholesterol oxidase and Chromogens are used to regulate the reaction. The intensity of the color formed is directly proportional to the HDL cholesterol concentration in the sample. Other non-HDL lipoproteins (LDL, VLDL and chylomicrons) It is prevented from reacting with enzymes with detergents. REAGENT PREPARATION Reagent 1 and Reagent 2 are ready to use. PRECAUTIONS: 1. For in vitro diagnostic use. 2. Do not pipette by mouth. 3. All specimens used in this test should be considered potentially infectious. Universal precautions as they apply to your facility should be used for handling and disposal of materials during and after testing. 4. Do not use the reagent after the expiration date printed on the kit. INTERFERENCES Bilirubin: No interference up to 40 mg/dL. Hemoglobin: No interference up to 300 mg/dL. Lipemia: No interference in the presence of triglycerides up to 1000 mg/dL. Ascorbic acid: No interference up to 5 mg/dL. Materials Required but not Provided 1. HDL/LDL Cholesterol Calibrator. 2. HDL cholesterol controls 3. Automated clinical chemistry analyzer capable of accommodating two reagent assays. PROCEDURE Primary Wavelength : 600 nm Secondary Wavelength : 700 nm Working temperature : 37°C