For the in vitro quantitative determination of albumin concentration in serum.
METHODOLOGY
At a controlled pH, bromcresol green forms a colored complex with albumin. The intensity of color at 630 nm is directly proportional to albumin content. The instantaneous initial absorbance is obtained as suggested by Webster
PRINCIPLE Controlled pH
BCG+ ALBUMIN Green BCG/ALBUMIN Complex
REAGENT COMPOSITION
Active Ingredients Concentrations
Bromcresol green 0,25 mM
Succinat Buffer 85 mM
Surfactant
PH 4.20 ± 0.1
PRECAUTIONS
1. For in vitro diagnostic use only.
2. DO NOT pipette by mouth. Avoid contact with skin and eyes. If spilt, thoroughly, wash affected areas with water.
3. Reagent contains Sodium Azide as a preservative. This may react with copper or lead plumbing to form explosive metal azides. Upon disposal, flush with large amounts of water to prevent azide build up.
4. Do not use the reagent after the expiration date printed on the kit. R37/38 Irritation to respiratory system and skin. R41 Causes serious eye damage In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.Wear eye/face protection.
REAGENT PREPARATION
Reagent is supplied ready to use.
REAGENT DETERIORATION
The reagent should not be used if: 1. The reagent is turbid. 2. The reagent fails to meet stated parameters of performance
SPECIMEN COLLECTION AND HANDLING
Collection: - No special preparation of the patient is necessary and sample preservatives are not required.