Alkaline phosphatase reagent kit ALP-10 series

solutionpreservativediagnostic

For the in vitro quantitative determination of Alkaline Phosphatase in human serum. METHODOLOGY Alkaline phosphatase in serum is determined by measuring the rate of hydrolysis of various phosphate esters under specified conditions. REAGENT COMPOSITION REAGENT 1 Diethanolamine 1.0 mM Magnesium chloride 0.5 mM REAGENT 2 p -Nitrophenylphosphate 10.0 mM pH 10.4 ± 0.2 Precautions and Warnings: 1. For in vitro diagnostic use only. 2. DO NOT pipette by mouth. Avoid contact with skin and eyes. If spilt, thoroughly wash affected area with water 3. Reagent contains Sodium Azide as a preservative. In a dry state may react with copper or lead plumbing to form explosive metal azides. Upon disposal, flush with large amounts of water to prevent azide build up. 4. Do not use the reagent after the expiration date printed on the kit. Reagent 1 contains Diethanolamine. R48/22: Harmful: danger of serious damage to health by prolonged exposure if swallowed. R41 : Causes serious eye damage. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.Wear suitable gloves and eye/face protection. If swallowed, seek medical advice immediately and show this container or label. REAGENT PREPARATION Reagents are ready to use, liquid form. Working Reagent : The reagents can be combined to make a working solution by mixing 4 parts of Reagent 1 with 1 part of Reagent 2 (e.g., 20mL Rgt 1 to 5 mL Rgt 2) REAGENT DETERIORATION DO NOT USE THE REAGENT IF: 1. The reagent is turbid and presence particles. 2. The reagent has an optical density greater than 1.3 at 405 nm