Uric acid reagent kit URI-10 series

solutionpreservativediagnostic

For the in vitro quantitative determination of Uric Acid in serum. METHODOLOGY This method uses uricase, peroxidase and DHBS the chromogen to yield a colorimetric end product. The colorimetric end product produced in this reaction can be measured at 520nm and is proportional to the uric acid concentration in the sample. Principle Uric Acid present in the sample originates a coloured complex according to the following reactions; Uric acid is converted by uricase into allantoin and hydrogen peroxides.The hydrogen peroxide initiates the coupling of 4-Aminoantipyrine to 3,5 dichloro2-hydroxybenzene sulfonic acid(DHBS) to form the chromogene which is measured at 520 nm and which is proportional to the amount of hydrogen peroxide generated from uric acid Precautions: 1. This reagent is for in vitro diagnostic use only. 2. Reagent contains Sodium Azide as a preservative. 3. Serum specimen should be considered infectious and handled appropriately. REAGENT PREPARATION Reagent is ready to use form. REAGENT DETERIORATION Do not use the reagent if: 1. The reagent is turbid. 2. The reagent blank has an absorbance of 0.40 or greater at 520nm. 3. The reagent does not meet stated performance parameters. INTERFERENCE 1. Bilirubin and ascorbic acid can result in falsely depressed uric acid levels. 2. Lipemic samples may cause falsely elevated uric acid levels. 3. Collection tubes containing formaldehyde as a presrevative must be avoided.