Urea reagent kit URE-11 series

solutionfor biochemistrydiagnostic

For the quantitative determination of UREA in serum. . For in vitro diagnostic use only. Methodology Talke and Schubert introduced a totally enzymatic procedure in 1965 utilizing urease and glutamate dehydrogenase.The present procedure is based on a modification of their method. Reagent Preparation Prepare working reagent by mixing 4 parts reagent 1 with 1 part reagent 2 (e.g.200 µL R1 with 50 µL R2 reagent) Reagent Deterioration The reagent should not be used if the working reagent has a reagent blank absorbance less than 1.0 at 340 nm. Precautions 1. This reagent is for in vitro diagnostic use only. 2. Avoid ingestion of reagent as toxicity has not yet been determined. 3. Reagents contain sodium azide (0.2%) as preservative Specimen Collection and Storage 1. Serum is recommended. 2. Plasma containing anticoagulants should not be used. 3. All material coming in contact with the sample must be free of ammonia and heavy metals. 5. All blood samples should be considered potentially infectious. Materials Required but not Provided 1. Accurate pipetting devices. (10ul and 1.0ml) 2. Timer. (Able to measure 30 and 60 second intervals) 3. Test tubes 4. Spectrophotometer with a temperature controlled cuvette able to measure at 340nm PROCEDURE Wavelength : 340 nm Temperature : 37°C Opthical path : 1 cm Assay Type : Fixed time Reaction Direction : Decreasing 1. Bring to room temperature ( 15 -30 °C) 2. Set the photometer to 0 (zero ) with distilled water. Limitations Samples with values above 250 mg/dL should be diluted with 0.9% saline 1:1, reassayed and the results multiplied by two.