Sodium reagent kit SOD-10 series

solutiondiagnosticlaboratory

For the quantitative determination of Sodium in human serum and plasma. METHODOLOGY Sodium is determined enzymatically via sodium dependent β -galactosidase activity with ONPG as substrate.The absorbance at 405 nm of the product O-nitrophenyl is proportional to the sodium concentration. Precautions: 1. This reagent is for in vitro diagnostic use only. 2. DO NOT INGEST. Avoid contact with skin and eyes. 3. Do not pipette by mouth. Reagent R1 and R2 contain Proclin 300. Avoid ingestion or contact with skin or mucous membranes. In case of skin contact, flush affected area with copious amounts of water. REAGENT PREPARATION All the reagents are ready to use. REAGENT DETERIORATION Do not use the reagent if: Presence of particles and turbidity. SPECIMEN COLLECTION AND STORAGE For use non-hemolysed serum . INTERFERANCES The following substances normalIy present in serum produced less than 10% deviation at the listed concentrations: NH4CI at 1,5 mM, KPi at 2.0 mM, CaCl2 at 7.5 mM, KCl at 10 mM, CuCl2bat 0.5 mM, ZnCI2 at 0.5 mM, FeCI3 at 0.5 mM, Glucose at 5 mM, ascorbate 10 mM, bilirubin at 40mg/dL, bilirubin conjugate 40 mg/dL, hemoglobin 500 mg/dL, and triglyceride 1000 mg/dL. Materials Required but not Provided 1. Spectrophotometer or colorimeter measuring at 405 nm. 2. Matched cuvettes 1.0 cm light path. 3. General laboratory equipment. PROCEDURE Wavelength : 405 nm Assay Type : Fixed time Reaction Direction : Increasing Temperature : 37°C Opthical path : 1 cm 2. Set the photometer to 0 (zero ) with distilled water 3. Mix and incubate for 7 minutes at 37° C and read the absorbance immediately (Abs.1)