Phosphorus reagent kit PHO-10 series

solutionfor biochemistrydiagnostic

For the in vitro quantitative determination of Inorganic Phosphorus in serum. METHODOLOGY The measurement of Inorganic phosphorus in serum is usually accomplished by forming a phosphomolybdate complex and in turn reducing it to a molybdenum blue color complex. Methods differ as to the choice of reducing agents: stannous chloride, phenylhydrazine, aminonaphtholsulfonic acld, ascorbic acid, pmethylaminophenolsulfate, N-phenyl-p-phenylenediamine and ferrous sulfate. These methods suffered from color instability, deproteinization steps and complexity of performance. The addition of a surfactant eliminated the need to prepare a protein-free filtrate, accelerated color production, stabilized the color and simplified the procedure. Many of the components in these reagents were unstable and had to be stored separately. The quantitative measurement of unreduced phosphomolybdate complexes was first reported by Simonsen in 1946. Daly and Ertingshausen adapted that technique for the determination of Inorganic phosphorus in 1972. Amador and Urban modified this procedure further the same year. The present method is a modification of the above procedure using a single, stable reagent performing in the UV range. Principle Inorganic Phosphorus + H2S04 + Ammonium Molybdate Unreduced Phosphomolybdate Complex. Inorganic phosphorus reacts with ammonium molybdate in an acid medium to form a phosphomolybdate complex which absorbs light at 340 nm. The absorbance at this wavelength is directly proportional to the amount of inorganic phosphorus present in the sample. Precautions 1. This reagent is for in vitro diagnostic use only.