Antibody reagent kit CKMB-30 series

for clinical chemistrycreatine kinase

Quantitative determination of creatin kinase MB (CK-MB) in human serum . PRINCIPLE OF THE METOD The procedure involves measurement of CK activity in the presence of an antibody to CKM monomer. This antibody completely inhibits the activity of CK-MM and half of the activity of CK-MB while not affecting the B subunit activity of CK-MB and CK-BB. Then it’s used the CK method to quantitatively determine CK-B activity. The CK-MB activity is obtained by multiplying the CK-B activity by two. CLINICAL SIGNIFICANCE CK-MB is an enzyme formed by the association of two subunits from muscle (M) and nerve cells (B). CK-MB is usually present in serum at low concentration; it is increased after an acute infarct of myocardium and later descends at normal levels. Also is increased, rarely, in skeletal muscle damage.Clinical diagnosis should not be made on a single test result; it should integrate clinical and other laboratory data REAGENT PREPARATION Reagents are supplied in a two vial, ready to use, liquid form. For some analyzers, the reagents can be combined to make a working solution by mixing 4 part of Reagent 1 with 1 part of Reagent 2 (e.g., 20mL R1 + 5mL R2). Precautions: Reagent 1 and Reagent 2: H360d May damage fertility or the unborn child P308+313 If exposed or concerned:get medical advice /attention REAGENT DETERIORATION Do not use the reagent if: the reagent blank absorbance < 0,300 at 340 nm against water. ADDITIONAL EQUIPMENT REQUIRED BUT NOT PROVIDED 1. A clinical chemistry analyzer capable maintaining constant temperature 37°C, and measuring absorbance at 340nm. 2. Deionized water and related equipment, e.g.: pipettes