Glucose reagent kit GLU -10 series

solutionfor clinical chemistrydiagnostic

For the in vitro quantitative determination of Glucose in serum. METHODOLOGY Early enzymatic methods for glucose determination used Glucose Oxidase to catalyze the oxidation of glucose to hydrogen peroxide and gluconic acid. The hydrogen peroxide that is formed is measured by the oxidation of a chromagens. Many chromagens were investigated but many were discarded because of possible carcinogenicity, toxicity, instability or because they were affected by many interfering substances. Trinder modified Emerson to develop an efficient peroxidase phenolaminophenazone system for the quantitation of hydrogen peroxide by formulation of a red quinoneimine dye. This method is less influenced by interfering substances and does not suffer from the many drawbacks of earlier methods. The present procedure is based on the above principle but utilizes a non-corrosive phenol substitute for added safety and convenience. PRECAUTIONS 1. This reagent is for in vitro diagnostic use only. 2. This reagent contains sodium azide at 0,05% REAGENT PREPARATION Reagent is liquid and ready to use. REAGENT DETERIORATION Do not use if: 1. The reagent fails to recover stated control values or meet stated linearity. 2. The reagent develops turbidity, or other evidence of microbial growth. INTERFERENCES Bilirubin: No interference up to 10 mg/dL. Hemoglobin: No interference up to 500 mg/dL. Lipemia: No interference in the presence of triglycerides up to 1500 mg/dL. Ascorbic acid: No interference up to 10 mg/dL. Grossly lipemic or icteric samples will cause false glucose values, consequently a patient blank should be run.