Solution reagent kit IGA series

for clinical chemistryIgA

For quantitative determination of immunglobulin A in human serum or plasma . Principle: The IgA is a quantitative turbidimetric test for the measurement of IgA in human serum or plasma. Anti human IgA antibodies when mixed with samples containing IgA, form insoluble complexes.These complexes cause an absorbance change, dependent upon the IgA concentration of the patient sample,that can be quantified by comparison from a calibrator of known IgA concentration. REAGENTS R1 (DILUENT): Tris buffer 20 mmol/L,PEG 8000 , pH 8.3. Sodium azide 0.95 g/L. R2 (ANTIBODY): Goat serum, anti human IgA . pH 7.5. Sodium azide 0.95 g/l CALIBRATION (Optional) Serum Proteins Calibrator is recommended. REAGENT PREPARATION Reagents are ready to use. PROCEDURE Wavelength: 600 nm (580-620) Working temperature: 37°C Optical path 1 cm Assay type: Turbidimetry Direction: Increasing 1. Bring the reagents and the photometer (cuvette holder) to 37°C . 2. Adjust the instrument to zero with distilled water. 3. Pipette into a test tube 4. Mix and read the absorbance (Abs.1) after the sample or calibrator addition. 5. Pipette into a test tube