IgM reagent kit IGM-30 series

solutionfor clinical chemistryIgA

For quantitative determination of immunglobulin IgM in human serum or plasma. PRINCIPLE OF THE METHOD The IgM is a quantitative turbidimetric test fort he measurement of IgA in human serum or plasma. Anti human IgM antibodies when mixed with samples containing IgM ,form insoluble complexes. These complexes cause an absorbance change ,dependent upon the IgM concentration of the patient sample,that can be quantified by comparison from a calibrator of known IgM concentration. REAGENTS R1 (DILUENT): Tris buffer 20 mmol/L, PEG 8000, pH 8.3. Sodium azide 0.95 g/L. R2 ((ANTIBODY): Goat serum, anti human IgM pH 7.5. Sodium azide 0.95 g/L. CALIBRATION (Optional) Serum Proteins Calibrator is recommended. REAGENT PREPARATION Reagents are ready to use. INTERFERENCES Bilirubin (20 mg/dL), hemoglobin (10.0 g/dL), lipemia (5g/L) do not interfere. Rheumatoid factor may interfere at 900 IU/mL. PROCEDURE Wavelength : 340 nm(320-360 nm) Working temperature : 37°C Optical path : 1 cm Assay type : Turbidimetry Direction : Increasing 1. Bring the reagents and the photometer (cuvette holder) to 37°C. 2. Adjust the instrument to zero with distilled water. 3. Pipette into a test tube. 4. Mix and read the absorbance (Abs.1) after the sample or calibrator addition. 5. Pipette into a test tube 6. Mix well and read the absorbance (Abs.2) of calibrators and sample exactly 2 minutes after the R2 addition. CALCULATION Calculate the absorbance difference (Abs.2-Abs.1) of each point of the calibration curve and plot the values obtained against the IgM concentration of each calibrator dilution.