Rheumatoid factor reagent kit RF-30 series

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Quantitative determination of Rheumatoid Factors (RF). CLINICAL SIGNIFICANCE Rheumatoid factors are a group of antibodies known RF concentration directed to determinants in the Fc portion of the immunoglobulin G molecule. Although rheumatoid factors are found in a number of rheumatoid disorders, such as systemic lupus erythematosus (SLE) and Sjögren’s syndrome, as well as in nonrheumatic conditions, its central role in clinic lies its utility as an aid in the diagnosis of rheumatoid arthritis. Principle: The RF-Turbilatex is a quantitative turbidimetric test for the measurement of RF in human serum or plasma. Latex particles coated with human gammaglobulin are agglutinated when mixed with samples containing RF. The agglutination causes an absorbance change, dependent upon the RF contents of sample that can be quantified by comparison from a calibrator of known RF concentration. PRECAUTIONS Components from human origin have been tested and found to be negative for the presence of HBsAg, HCV, and antibody to HIV (1/2). However handle cautiously as potentially infectious. CALIBRATION bt products recommends using RFCAL-30 calibrator . Recalibrate when control results are out of specified tolerances, when using different lot of reagent and when the instrument is adjusted. REAGENT PREPARATION Reagents are supplied in a two vial, ready to use, liquid form. Swirl the latex vial gently before use. For some analyzers, the reagents can be combined to make a working solution by mixing 4 part of Diluent (R1) with 1 part of Latex (R2) (e.g., 4mL R1 + 1mL R2).