Protein ladder Blu10 Plus

for Western blot

The Blu10 Plus Prestained Protein Ladder is a combination of 10 pre-stained proteins with molecular weights from 6.5 to 270 kDa. The 10 recombinant proteins are covalently coupled with blue chromophore, while 2 orange bands at 30 kDa and 270 kDa and a green band at 52 kDa serve as reference bands. The Blu10 Plus Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes. -Guide for Molecular Weight Estimation (kDa) Migration patterns of Blu10 Plus Prestained Protein Ladder in different electrophoresis conditions are listed below : 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring. Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment. Nowadays, more and more researchers are studying the high molecular weight proteins, meaning they may need high-resolution prestained protein standards, especially with high molecular weights. That's why we're pleased to launch the color-enhanced Blu10 Plus prestained protein standards. As you may see the comparison data shown below, Blu10 Plus shows a better resolution than the Blu12 & Blu10 for the protein bands from 95 kDa to 270 kDa. Required materials but not provided: Vertical Electrophoresis system Power supplies Vortex or equivalent Microcentrifuge