Protein ladder Blu10
for Western blotureapH

Protein ladder - Blu10 - Bio-Helix - for Western blot / urea / pH
Protein ladder - Blu10 - Bio-Helix - for Western blot / urea / pH
Add to favorites
Compare this product

Characteristics

Type
protein ladder
Applications
for Western blot
Tested parameter
pH, glycerol, urea
Storage temperature

Min.: -20 °C
(-4 °F)

Max.: 25 °C
(77 °F)

Description

The Blu10 Prestained Protein Ladder is a combination of 10 pre-stained proteins with molecular weights from 11 to 180 kDa. The 10 recombinant proteins are covalently coupled with blue chromophore, while 1 green band at 25 kDa and a red band at 75 kDa, serve as reference bands. The Blu10 Prestained Protein Ladder keeps track of the size and separation of proteins during SDS-polyacrylamide gel electrophoresis, approximating protein size, and validating Western transfer efficiency on PVDF, nylon, or nitrocellulose membranes. -Guide for Molecular Weight Estimation (kDa) Migration patterns of Blu10 Prestained Protein Ladder in different electrophoresis conditions are listed below : 3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively. 2.5 μl per well for general Western transferring. Note: The molecular weight of each protein (kDa) was measured against an unstained protein ladder in every electrophoresis condition. Additional data should be considered for a more accurate adjustment. Storage Buffer : Approximately 0.2~0.4 mg/ml of each protein in buffer (20 mM Trisphosphate pH 7.5 at 25°C), 2% SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15% (v/v) Glycerol. Storage : Stable for up to 2 weeks at 25°C. Stable for up to 3 months at 4°C. Stable for up to 24 months at -20°C.

VIDEO

Catalogs

No catalogs are available for this product.

See all of Bio-Helix‘s catalogs
*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.