Solution reagent UltraScence Pico Plus

antibodyantigenfor Western blot

The UltraScence Pico Plus Western Substrate, as a luminol-based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The low picogram to high femtogram detection of antigen is enabled by UltraScence Pico Plus Western Substrate’s excellent sensitivity and long signal duration. Further, its long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration. - No optimization required. Switching to the UltraScence Pico Plus Western Substrate from other brands, such as Thermo Scientific™ SuperSignal™ Pico Plus and Bio-Rad™ Clarity™, does not require optimization or protocol changes. - High degree of sensitivity and enhanced chemiluminescence duration. UltraScence Pico Plus Western Substrate enables an accurate low picogram to high femtogram detection of protein on the same immunoblot after a single exposure. - Optimized for use with PVDF and nitrocellulose membranes. - Compatible with Western Blotting Markers. - Optimized for film and CCD-based imaging. 1. Keep the membrane moist in the wash buffer while preparing the substrate mixture. Make sure the membrane does not dry out in the next steps. 2. The chemiluminescent substrate solution is sufficiently stirred to prepare the 0.1ml of solution / cm2 of the membrane. - For a small membrane (7 x 8.5 cm), 4 ml of solution is sufficient. - For a medium-sized membrane (8.5 x 13.5 cm), 10 ml of solution is sufficient.