Solution reagent UltraScence Pico Plus

antibodyantigenfor Western blot

The UltraScence Pico Plus Western Substrate Powder, as a luminol-based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The low picogram to high femtogram detection/low picogram to mid femtogram detection of antigen is enabled by UltraScence Pico Plus-Pico Ultra/Femto Western Substrate’s excellent sensitivity and long signal duration. Further, its long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration. -Ready-to-ship and significantly longer shelf life in the powder form! -Significant reductions in transportation and storage costs, resulting in less carbon footprint! -Empower custom production anywhere in the world! -Significantly more sensitive than leading manufacturers on the market. Membranes were probed with Rabbit anti β-actin antibody (GTX109639) diluted at 1:5,000 of and then with Goat anti Rabbit HRP-conjugated secondary antibody (GTX213110-01, 1:10,000) after serial dilution mock RD cell lysates were prepared and applied in electrophoresis and protein transfer. Identical blots were incubated with 400 μL of UltraScence Western substrate. The blots were simultaneously exposed for 30 seconds, 60 seconds and 180 seconds using Chemlux SPX-600 Series digital imaging system. The Luminol and Peroxide solution of UltraScence Pico Plus were mixed in a 1:1 ratio and having 400 uL treated on each blot after taken out from 4℃ for 1hr, 12 hrs and 24 hrs.