Solution reagent UltraScence Femto Plus

antibodyantigenIgG

The UltraScence Femto Plus Western Substrate Powder, as a luminol-based enhanced chemiluminescent substrate, is sensitive and compatible with conducting immunoblots with horseradish peroxidase (HRP) – conjugated secondary antibodies. The mid femtogram to low femtogram detection of antigen is enabled by UltraScence Femto Plus Western Substrate’s excellent sensitivity and long signal duration. Further, its long chemiluminescent signal duration makes both digital and film-based imaging possible without any loss of the signal. Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration -Ready-to-ship and significantly longer shelf life in the powder form! -Significant reductions in transportation and storage costs, resulting in less carbon footprint! -Empower custom production anywhere in the world! -Significantly more sensitive than leading manufacturers on the market. The Luminol and Peroxide solution of UltraScence Femto Plus were mixed in a 1:1 ratio and having 400 uL treated on each blot after taken out from 4℃ for 1hr, 12 hrs and 24 hrs. The blots were simultaneously exposed for 5, 10, 30, 60, and 180 seconds using Chemlux SPX-600 Series digital imaging system. The EGFP (Enhanced Green Fluorescent Protein) was serially diluted for electrophoresis, after the proteins were transferred to the membrane, they were blocked and probed with GFP tag Rabbit PolyAb diluted solution (1:10,000), and followed by the Goat Anti-rabbit IgG/HRP secondary antibody (1:10,000).