Solution reagent PS002-L05X

staining solutiondyeprotein ladder

Innovatively developed Next-Generation Protein Stain for faster and environment-friendly gel visualization. 5X COOMASSIE nano, innovatively enhanced by the proprietary nano-technology, is a concentrated, easy-to-reconstitute protein staining solution for SDS-PAGE gels and is also compatible with mass spectrophotometry. Its next generation formula offers faster protein detection and higher sensitivity while eliminating the conventional need for destaining and wash. In the absence of hazardous substances, such as methanol and acetic acid, 5X COOMASSIE nano is considered to be safe and environmentally friendly by reducing carbon footprint by as much as 80%. COOMASSIEnano Protein Staining Solution practices the fast-signal representing at 5 min. Prestained protein ladder, unstain protein ladder, and BSA were prepared and applied in electrophoresis. After running SDS-PAGE (4-20% Novex Gel), please remove the gel from the cassette then proceed to submerge the gel in proper amount of COOMASSIEnano dye, enough to cover the gel. Lightly agitate the staining box for 5 minutes to over night at room temperature. COOMASSIEnano Protein Staining Solution demonstrates the high sensitivity detection could be down to 10 ng (Lane H). Prestained protein ladder, unstained protein ladder, and serial diluted BSA were prepared and applied in electrophoresis. After running 10% homemade gel (0.75 mm thickness), please remove the gel from the cassette then proceed to submerge the gel in a proper amount of COOMASSIEnano Protein Staining Solution, enough to cover the gel. Lightly agitate the staining container at room temperature when staining for 30 hrs.