DNA ladder RScript

reverse transcriptasediagnosticRT-qPCR

Overcome the most challenging RNA structures over a wide temperature range. Bio-Helix RScript Reverse Transcriptase - Engineered innovatively and specifically for both Research and Diagnostic applications for meeting all your cDNA synthesis needs and for overcoming the most challenging secondary RNA structures over a wide temperature range. Our next-generation, engineered recombinant M-MLV reverse transcriptase, with improved thermostability, processivity, robustness, optimal cDNA yields, proprietary site mutations for reduced RNase H activity, and extended half-life, is the most versatile reverse transcriptase in the world for not only simply meeting the routine cDNA synthesis requirements but also enabling superior performance for even the most challenging RNA samples at hand. Stability of RScript reverse transcriptase: The stability RT-qPCR data shows the performance of RScript reverse transcriptase is maintained without any significant alteration in performance at 37 °C after a period of 3 weeks (21 days), demonstrating its high resistance to temperature and time. The percentage of RScript reverse transcriptase activity was calculated by dividing values at each reaction temperature. Enhancement of the thermostability: Reverse transcription of a 1 kb fragment ARHGAP29 RNA using RScript reverse transcriptase or competitor reverse transcriptases were used to carry this experiment. Regardless of the variations in temperatures (42 -60 °C) RScript reverse transcriptase shows a stable and superior performance at higher temperatures of up to 60 ℃ compared to other brands. The molecular weight marker used was DNA Ladder 1kb #SDL-1000R.