Histology reagent 05-20006/L

liquid

Primary container: white bottle in High Density Polyethylene (HDPE). Useful capacity 1 l. HDPE cap. Tamper evident cap. Wear, water, alcohol and solvents resistant PVC label. Scratchproof ink resistant to water and alcohol. Expected aim Product for the preparation of cyto-histological samples for optical microscopy. Application Mordant solution used in the Gram procedure in tissue sections. Principle Two dyes are used one after the other: crystal violet and phloxine B. Crystal violet solution precipitates through oxidation with a iodine solution. The deriving complex attaches to bacteria cell walls with bonds of varying nature and intensity. The differentiating solution removes the crystal violet-iodine complex from the walls of some bacteria, but it does not act on others. These retain the primary dye and are called Gram-positive. Decolorized bacteria are then counterstained with a red dye; they are called Gram-negative. Method 1)Section to distilled water 2)Phloxine B solution 15 minutes at 56-58°C 3)Rinse in distilled water 4)Crystal violet solution3 minutes 5)Iodine solution3 minutes 6)Rinse in distilled water 7)Dry in the air 8)Acetone-limonene1 minute 9)Acetone-limonene 1 minute Results Gram-positive bacteria .......................blue Gram-negative bacteria ..................... red Warning and precaution The product must be used exclusively by specialized technical operators. Carefully read the information on the classification of dangerous substances on the label.