Histology reagent 05-30010/L

liquid

Primary container: white bottle in High Density Polyethylene (HDPE). Useful capacity 1 l. HDPE cap. Tamper evident cap. Wear, water, alcohol and solvents resistant PVC label. Scratchproof ink resistant to water and alcohol. Expected aim Product for the preparation of cyto-histological samples for optical microscopy. Application Decolorizing solution used in Gram’s staining. Principle Two dyes are used one after the other: crystal violet and safranine. Crystal violet solution precipitates through oxidation with a iodine solution. The deriving complex attaches to bacteria cell walls with bonds of varying nature and intensity. The Gram's decolorizing solution removes the crystal violet-iodine complex from the walls of some bacteria, but it does not act on others. These retain the primary dye and are called Gram-positive. Decolorized bacteria are then counterstained with a red dye; they are called Gram-negative. Method For smears 1)Air dry the smear 2)Crystal violet solution according to Hucker 1 minute 3)Wash in distilled water 4)Gram’s iodine 1-2 minutes 5)Wash in distilled water6)Gram’s decolorizing solution 1 minute 7)Wash in distilled water 8)Safranine solution 1 minute 9)Wash in distilled water 10)Air dry the smear 11)Examine under the microscope in immersion oil Results Gram-positive bacteria..................................................................blue-violet Gram-negative bacteria .................................................................red-orange Storage Store the preparation at room temperature. Keep the containers tightly closed.