Staining solution reagent Afog

for histologyfor cytology

Minimum number of tests that can be performed 100 Completion time 22 minutes Shelf life 2 years Storage conditions 15-25°C Additional equipment Not required Application Reference method for highlighting protein deposits in renal biopsy. Recommended fixative: Bouin. Result Collagen fibrils blue Nuclei black Erythrocytes, cytoplasm pink - orange Elastic fibers pale pink - yellow or colorless Protein deposits bright red Product for the préparation of cyto-histological samples for optical microscopy. Standard procedure for visualisation of glomerular protein deposits in kidney biopsy; recommended fixative: BOUIN PRINCIPLE In this method, three different dyes are used: Weigert hematoxylin for nuclear staining, orange G for cytoplasm and aniline blue for a sélective collagen staining. Selectivity in this procedure is due to different degrees of affinity between dyes and tissue macromolecules. A central rôle is played by phosphomolibdic acid, which acts as a bound between tissue structures (collagen fibrils, cell membranes), and aniline blue (amphoteric dye). Orange G, which is the second component of AFOG solution, has no affinity to phosphomolibdic acid and is thus used to stain ail remaining structures. Acid fuchsin stains selectively glomerular protein deposits. The product is intended for professional laboratory use for healthcare professionals. Carefully read the information on the label (danger symbols, risk and safety phrases) and always consult the safety data sheet. Do not use if the primary container is damaged. In the event of a serious accident, we recommended that you immediately inform Bio-Optica Milano S.p.A and the competent authorities.