Staining solution reagent AgNOR

for histologyfor cytology

Minimum number of tests that can be performed 12 preparations (up to 4 slides per preparation) Completion time 30 minutes Shelf life 1 year Storage conditions 15-25°C Additional equipment glass rod, jars for washing in distilled water Application Method for highlighting argentaffin proteins (100 kD) in the nucleolus organizer region (NOR) on paraffin-embedded sections and smears. Result Product for the préparation of cyto-histological samples for optical microscopy. To show argentaffine proteins (100 KD), which are présent in nucleolar organizer région (NOR), in smears and sections embedded in paraffin. PRINCIPLE Nucleolar proteins 100 KD acts with RNA polymerase I to form AgNOR that is proteins bound to NOR which can be showed by silver imprégnation. Argyrophilic reaction is due to the intrinsic capacity of 100 KD to act as a reducing substance on silver ions in the solution, binding them in the reduced form of metallic silver. Just before use, the silver nitrate solution is mixed with positively charged gélatine aqueous solution that favours the contact between reducing groups of nucleolar proteins and silver ions. WARNING - Always use excellent distilled water to wash the slides. - Do not use poly-lysine coated glass slides. - Avoid the use of metallic object (tweezers, slide racks etc.) - After mounting, keep the slides in a dark place. - If reagent A, stored at lower temperature than advised one, results in gel form can be fluidified bringing bottle under warm tap water