Minimum number of tests that can be performed 100
Completion time 50 minutes
Shelf life 2 years
Storage conditions 15-25°C
Additional equipment Not required
Application
Method indicated for highlighting acid mucopolysaccharides on tissue sections.
Result
Acid mucopolysaccharides blue - turquoise
Nuclei red
Method
1) Bring the section to the distilled water.
2) Dispense 10 drops of reagent A onto the section: leave to act for 3 minutes.
3) Without washing, drain the slide and dispense 10 drops of reagent B onto the
section: leave to act for 30 minutes.
4) Without washing, drain the slide and dispense 10 drops of reagent C onto the
section: leave to act for 10 minutes.
5) Wash in distilled water.
6) Dispense 10 drops of reagent D onto the section: leave to act for 5 minutes.
7) Wash in distilled water.
8) Dehydrate by means of the ascending series of alcohols; xylene and balsam