Staining solution reagent Alcian blue pH 2,5 PAS

for histologyfor cytology

Minimum number of tests that can be performed 100 Completion time 1 hour 25 minutes Shelf life 1 year Storage conditions 2-8°C Additional equipment Not required Application Combined method for differentiating acid mucins, neutral mucins and carbohydrates on tissue sections. Result PAS-positive substances magenta red Acid mucopolysaccharides blue - turquoise Certain acid mucins and cartilage from purple to dark blue METHOD 1) Bring section to distilled water. 2) Put on the section 10 drops of reagent A: leave to act 30 minutes. 3) Drain the slide without washing and put on the section 15 drops of reagent B: leave to act 10 minutes. 4) Wash for 5 minutes in tap water and for 2 minutes in distilled water. 5) Put on the section 10 drops of reagent C: leave to act 10 minutes. 6) Wash in distilled water. 7) Put on the section 10 drops of reagent D: leave to act 20 minutes. 8) Wash in distilled water. 9) Put on the section 10 drops of reagent E: leave to act 2 minutes. 10) Drain the slide without washing and put on the section 10 drops of reagent F: leave to act 3 minutes. 11) Wash in distilled water. 12) Put on the section 10 drops of reagent G: leave to act 2 minutes. 13) Blue in running tap water for 5 minutes. 14) Dehydrate through ascending alcohols; clear in xylene and mount.