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Stain reagent Azan trichrome
staining solutionfor histologyfor histopathology

Stain reagent - Azan trichrome - BIO-OPTICA Milano - staining solution / for histology / for histopathology

Stain reagent - Azan trichrome - BIO-OPTICA Milano - staining solution / for histology / for histopathology

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Go to the BIO-OPTICA Milano website for more information

Characteristics

Type

staining solution, stain

Applications

for histology, tissue, for histopathology, pathology

Format

liquid

Storage temperature

Max.: 25 °C
(77 °F)

Min.: 15 °C
(59 °F)

Description

Product identification
Azan trichrome histological staining kit
Product code: 04-001802
Pack size: 100 tests
Manufacturer: Bio-Optica

Key specifications

Intended use: routine histological connective tissue staining
Processing time: approx. 1 h 40 min (per run)
Shelf life: 2 years

Intended application and staining results
Preferred method for connective tissue sections; suitable for muscle fibers, glial elements, collagen, reticulum and glomerular stroma, erythrocytes and nuclear chromatin.

Collagen, reticulum, basophilic cytoplasmic granules: blue
Neurofibrils (neuroglia): reddish
Muscle: orange
Nuclei, erythrocytes, acidophilic pituitary granules: red
Delta‑cell cytoplasmic granules (pituitary): blue

Principle
The method combines cytological staining with an acid dye (azocarmine) and a contrasting aniline blue after mordanting with phosphotungstic acid. For optimal contrast, over-stain with azocarmine and differentiate slowly with anilinoalcohol to avoid predominance of the contrast stain.

Method (summary)

1) Hydrate the section in distilled water.
2) Incubate the section in reagent A in an oven at 56 °C for 30 minutes; remove and wait 5 minutes. Recover dye in vial A without filtering.
3) Rinse in distilled water.
4) Apply 10 drops of solution B to the section; leave for 1 minute.
5) Drain on filter paper and apply 10 drops of reagent C to the section; leave for 1 minute.
6) Drain on filter paper and apply 10 drops of reagent D to the section; leave for 30 minutes.

Required equipment and handling

Vertical histology staining rack or dish
Oven capable of 56 °C
Distilled water, filter paper, ethanol series for dehydration, xylene and mounting medium
Handle reagents with appropriate PPE; consult SDS before use

Documentation and conformity
Technical Datasheet languages

IT
EN
CS
DK
DE
EL
ES
ET
FI
FR
HR
HU
LT
LV
NL
PL
PT
RO
SK
SV
BG

Safety Datasheet (SDS) languages

IT
EN
DK
DE
EL
ES
ET
FI
FR
HU
LT
LV
NL
PL
PT
RO (RM)
SK
SV
NO
BL
CR
CZ
RU
TR

CE conformity
CE conformity document available (IT, EN).

Notes
Product marketed by Bio-Optica. Pack size indicated as 100 tests. Consult technical datasheet and SDS for complete handling, disposal and storage instructions.

Catalogs

General Catalogue

General Catalogue

139 Pages

Go to the BIO-OPTICA Milano website for more information

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