Staining solution reagent 04-001802

for histologyfor cytology

Minimum number of tests that can be performed 100 Completion time 1 hour 40 minutes Shelf life 2 years Storage conditions 15-25°C Additional equipment Vertical histology jar, oven Application The method of choice for connective tissue, particularly indicated for muscle and glial fiber, collagen, reticulum, glomerular stroma of the kidney, erythrocytes and nuclear chromatin on histological sections. Result Collagen, reticulum, basophilic cytoplasmic granules of the pituitary gland, juxtaglomerular granules and glomerular stroma of the kidney: blue Neurofibrils (glia): reddish Muscle: orange Nuclei, erythrocytes and acidophilic granules of the pituitary gland: red Cytoplasmic granules of the delta cells of the pituitary gland: blue PRINCIPLE In this method, two acid dyes are used: azocarmine and aniline blue. A cytological stain with azocarmine is combined with a counterstain with aniline blue, after treatment with phosphotungstic acid. To obtain good results, it is necessary to overstain with azocarmine, then slowly differentiate with aniline-alcohol to prevent counterstain from covering stained muscle, collagen etc. METHOD 1) Bring sections to distilled water. 2) Pour reagent A into a Coplin jar and incubate the sections at 56° C for 30 minutes. Allow to cool for 5 minutes at room temperature. Recover the dye in the bottle A without filtering. 3) Wash in distilled water. 4) Put on the section 10 drops of reagent B: leave to act 1 minute. 5) Drain the slide without washing and put on the section 10 drops of reagent C: leave to act 1 minute.