Staining solution reagent Brown-Brenn

for histologyfor cytology

Minimum number of tests that can be performed 100 Completion time 8 minutes Shelf life 2 years Storage conditions 15-25°C Additional equipment Not required Application Method for differentiating Gram-positive and Gram-negative bacteria on histological sections and smears. Result Gram-positive bacteria blue Gram-negative bacteria red Actinomycetes (Nocardia) blue Nuclei red Other tissue elements yellow Product for the preparation of cyto-histological samples for optical microscopy. For demonstration Gram-positive and Gram-negative bacteria in tissue sections and smears. PRINCIPLE Gram staining is the most important method to differentiate bacteria species. Two dyes are used one after the other: crystal violet and fuchsine. Crystal violet solution precipitates through oxidation with a iodine solution. The deriving complex attaches to bacteria cell walls with bonds of varying nature and intensity. The differentiating solution removes the crystal violet-iodine complex from the walls of some bacteria, but it does not act on others. These retain the primary dye and are called Gram- positive. Decolorized bacteria are then counterstained with a red dye; they are called Gram negative. Gram-positive bacteria’s capacity to retain the dye-iodine complex is usually ascribed to the bond which develops between the complex and a molecule only Gram-positive possess, namely magnesium ribonucleate. The product is intended for professional laboratory use for healthcare professionals. Carefully read the information on the label (danger symbols, Nrisk and safety phrases) and always consult the safety data sheet. Do not use if the primary container is damaged.