Staining solution reagent Dane

for histologyfor cytology

Minimum number of tests that can be performed 100 Completion time 40 minutes Shelf life 2 years Storage conditions 15-25°C Additional equipment Not required Application For viewing prekeratin, keratin and mucin on histological sections, therefore particularly indicated for skin pathology Result Prekeratin and keratin orange, red - orange Mucins turquoise Nuclei orange - brown Method 1) De-wax the section and bring it to the distilled water. 2) Dispense 10 drops of reagent A onto the section: leave to act for 10 minutes. 3) Leave to develop for 5 minutes in running tap water. 4) Dispense 10 drops of reagent B onto the section: leave to act for 3 minutes. 5) Wash in tap water for 3 minutes. 6) Dispense 10 drops of reagent C onto the section: leave to act for 5 minutes. 7) Wash in tap water. 8) Dispense 10 drops of reagent D onto the section: leave to act for 13 minutes. 9) Wash in distilled water and dehydrate rapidly by means of the ascending series of alcohols, stopping for 1 minute in the last absolute, xylene and balsam.