Staining solution reagent Diastase

for histologyfor cytology

Minimum number of tests that can be performed 40 Completion time 30 minutes Shelf life 1 year Storage conditions 2-8°C Additional equipment vertical jar Application Digestion on a histological section with a solution of diastase is always indicated when you want to remove the glycogen so as to observe only the neutral epithelial mucins. It is the method of choice in liver biopsy. Result The removal of glycogen can be detected, after PAS reaction, by comparing the sample section with an adjacent section of the same preparation that has not been treated with diastase. Product for the préparation of cyto-histological samples for optical microscopy. Digestion of histologie sections by means of diastase solution is advisable when the purpose is to remove glycogen in order to observe épithélial neutral mucins. This method is recommended for liver biopsy. PRINCIPLE The Diastase essentially hydrolyses starch and glycogen on the sections of tissue. Usually digestion of glycogen is not complété on tissue fixed because of particular glycosidic bond that they are formed during conventional fixation and it is possible to observe a granular glycogen precipitate to a pôle of the hépatocytes.