Staining solution reagent Colloidal iron

for histologyfor cytology

Minimum number of tests that can be performed 100 Completion time 1 hour 35 minutes Shelf life 2 years Storage conditions 15-25°C Additional equipment 50 ml vertical histology jar, graduated cylinder and glass rod Application Indicated method for viewing acid mucins. Specificity: the reaction shows acid mucins (sialomucins and sulfomucins) whose acid groups, at the reaction pH, take anionic form and are therefore capable of forming a stable complex with positive trivalent iron. Result Acid mucins blue Cellular nuclei red Product for the preparation of cyto-histological samples for optical microscopy. For demonstration of the acid mucins. Specificity: the reaction shows the acid mucins (sialo and sulphured mucins) which form a stable complex with trivalent iron thanks to the presence of their acid groups in anionic form. PRINCIPLE This method is based on the bond that take place in acid environment between colloidal iron and acid groups of the mucopolysaccharides. At these conditions, the iron forms a compound of chelation that is demonstrated by method of Bleu of Prussia: the potassium ferrocyanide reacts with ferric ions of compound of chelation in acid environment and it forms a coloured salt: Prussian blue. Reaction takes place, in ionic form as follow: 4 Fe++ + 3K4Fe(CN)6= Fe 4Fe(CN 6)3 + 12 K+