Staining solution reagent Feulgen

for cytologyfor histology

Minimum number of tests that can be performed 100 Completion time 1 hour 5 minutes Shelf life 1 year Storage conditions 2-8°C Additional equipment Not required Application The method is used to highlight deoxyribonucleic acid (DNA) on tissue sections. Result DNA magenta red Method 1) Bring the section to the distilled water. 2) Dispense 10 drops of reagent A onto the section, leave to act for 40 minutes. CAUTION: reagent A is corrosive. Handle with care and in an environment equipped with an extractor fan. Avoid contact with the skin and eyes. Wear protective gloves and goggles. 3) Wash twice in distilled water. 4) Dispense 10 drops of reagent B onto the section: leave to act for 10 minutes. 5) Without washing, drain the slide and dispense 10 drops of reagent C onto it: leave to act for 2 minutes. 6) Without washing, drain the slide and dispense 10 drops of reagent D onto it: leave to act for 3 minutes. 7) Wash in running tap water for 5 minutes. 8) Dehydrate by means of the ascending series of alcohols; xylene and balsam.