Staining solution reagent Fouchet Van Gieson

for histologyfor cytology

Minimum number of tests that can be performed 100 Completion time 35 minutes Shelf life 2 years Storage conditions 15-25°C Additional equipment Not required Application For highlighting bilirubin pigment on tissue sections. Result Bilirubin green Connective red Collagen yellow Product for the preparation of cyto-histological samples for optical microscopy. For demonstration of bilirubin and collagen in tissue sections PRINCIPLE The bilirubin is the derived without iron of the biliverdin. In its turn, the biliverdin is the result of the degradation of the haemoglobin. The reaction happens mainly in the bony marrow, the spleen and the liver. In the liver of patients with obstructive icterus, the bilirubin appears as thrombi in the biliary canaliculus and granules in hepatocytes and cytoplasm of the cells of Kupfer The pigment is insoluble in water and in the aqueous fixative, but it can change to the green colour in case of extended formalin fixation. The colour developed from Fouchet reaction is based on strong redox of the complex and consequent transformation in biliverdin. WARNING False positive reactions may be excluded with Perl's reaction, always negative for bilirubin METHOD 1) Bring sections to distilled water. 2) Put on the section 5 drops of Reagent A and 5 drops of Reagent B: let them work 5 min. 3) Rinse in distilled water. 4) Put on the section 10 drops of Reagent C, let it work 7 min. 5) Without rinsing, air - dry 5 min. 6) Dehydrate in absolute alcohol, clear in xylene and mount.