Stain reagent Fouchet Van Gieson

staining solutionfor histologytissue

Product
Fouchet Van Gieson staining kit; Product code: 04-121872; Pack size: 100 tests; Minimum number of tests: 100; Procedure time: 35 minutes; Shelf life: 2 years; Additional equipment: not required.

Application and results
Designed to demonstrate bilirubin pigment on tissue sections for optical microscopy.

• Result: Bilirubin green
• Connective tissue red
• Collagen yellow

Principle
Bilirubin is the iron-free derivative of biliverdin, produced by haemoglobin degradation by the reticulo‑endothelial system. The Fouchet reaction involves strong oxidation‑reduction of the complex and conversion to biliverdin, producing characteristic colour changes. The reaction occurs mainly in bone marrow, spleen and liver. Bilirubin is insoluble in water and aqueous fixatives and may turn green after prolonged formalin fixation.

Method

• 1) Hydrate sections in distilled water.
• 2) Apply 5 drops of reagent A to the section and add 5 drops of reagent B; incubate 5 minutes.
• 3) Rinse in distilled water.
• 4) Apply 10 drops of reagent C to the section; incubate 7 minutes.
• 5) Drain the slide without rinsing and dry first with filter paper then air‑dry 5 minutes.
• 6) Absolute alcohol 15 seconds, xylene and mount with balsam.

Documentation & conformity

• Technical datasheet: available in multiple languages (IT, EN, CS, DK, DE, EL, ES, ET, FI, FR, HR, HU, LT, LV, NL, PL, PT, RO, SK, SE, BL)
• Safety datasheet (SDS): available in multiple languages (IT, EN, DK, DE, EL, ES, ET, FI, FR, HU, LT, LV, NL, PL, PT, RO, SK, SE, NO, BL, CR, CZ, RU, TR)
• CE conformity: certificate available (IT / EN)
• Image: FouchetVanGieson.jpg