Staining solution reagent Gomori's paraldehyde fuchsin

for histologyfor cytology

Minimum number of tests that can be performed 100 Completion time 1 hour 15 minutes Shelf life 2 years Storage conditions 15-25°C Additional equipment Not required Application For viewing elastic fibers and secretory granules in alpha and beta cells of the islets of Langerhans of the endocrine pancreas. Results Pancreatic beta-cell granules dark violet Cellular nuclei dark violet Connective tissue red Tessuto connettivo green Product for the preparation of cyto-histological samples for optical microscopy. For demonstration elastic fibres, beta cells of pancreas, mast cells granules, mucin, argentaffin granules and acrosomes of spermatozoa. METHOD 1) Bring sections to distilled water. 2) Put on the section 5 drops of reagent A and 5 drops of reagent B: leave to act 10 minutes. 3) Rinse in distilled water. 4) Put on the section 10 drops of reagent C, leave to act 5 minutes. 5) Rinse in distilled water. 6) Put on the section 10 drops of reagent D: leave to act 5 minutes. 7) Without washing, drip the slide and insert it in the humid chamber, put on the section 10 drops of reagent E: leave to act 20 minutes. 8) Drip the slide and put on the section 10 drops of reagent F: leave to act 10 minutes. 9) Rinse in distilled water. 10) Put on the section 10 drops of reagent G: leave to act 10 minutes. 11) Rinse in distilled water. 12) Put on the section 10 drops of reagent H: leave to act 5 minutes. 13) Rinse in distilled water, dehydrate in alcohol 95, absolute alcohol, clear in xylene and mount.