Staining solution reagent Grimelius

for histologyfor cytology

Minimum number of tests that can be performed 100 Completion time 3 hours 35 minutes Shelf life 1 year Storage conditions 2-8°C Additional equipment Graduated cylinder, 50 ml histology jar, oven Application For viewing argyrophilic substances on tissue sections and appositions. Result Argyrophilic granules from light brown to black Product for the préparation of cyto-histological samples for optical microscopy. To show argyrophilic substances in tissue sections. PRINCIPLE This method is based on the intrinsic capacity of some tissue components to bind to silver halides. These are then revealed by a photographie process that reduces silver salts to metallic silver. Selectivity of method is due to a low concentration of silver salts in working solution. A second short imprégnation is used to precipitate more silver on the sites where it has already set down and therefore to make them blacker. Method 1) Bring the section to the distilled water. 2) Dispense 10 drops of reagent A onto the section, leave to act for 40 minutes. CAUTION: reagent A is corrosive. Handle with care and in an environment equipped with an extractor fan. Avoid contact with the skin and eyes. Wear protective gloves and goggles. 3) Wash twice in distilled water. 4) Dispense 10 drops of reagent B onto the section: leave to act for 10 minutes. 5) Without washing, drain the slide and dispense 10 drops of reagent C onto it: leave to act for 2 minutes. 6) Without washing, drain the slide and dispense 10 drops of reagent D onto it: leave to act for 3 minutes. 7) Wash in running tap water for 5 minutes. 8) Dehydrate by means of the ascending series of alcohols; xylene and balsam.