Staining solution reagent Grocott MW

for histologyfor cytology

Minimum number of tests that can be performed 120 Completion time 50 minutes Shelf life 1 year Storage conditions 2-8°C Additional equipment graduated cylinder, glass rod, oven Application Method used for viewing fungi on a tissue section. Result Fungi clearly outlined in black Mucins dark gray Background green Product for the preparation of cyto-histological samples for optical microscopy. To demonstrate fungi in tissue sections. PRINCIPLE In most fungi, cell walls are made of chitin, a polymer of N-acetylglucosamine together with polymers of D-glucose and D-mannose, proteins and lipids. Periodic acid reacts with glycolic and glycoaminic groups in polysaccharide chain oxidising them to aldehydic groups and thus breaking the chain itself. These newly formed aldehydic groups reduce silver chloride, which is part of the silver-methenamine complex, to metallic silver and make it visible. WARNING For good results, follow these rules: - Avoid to infect section and slide with no pathogenic fungi (handle only with gloves, do not expose preparation to air) - Use always fresh distilled water. - Use only clean glasswork. - Avoid deposit of dust on section. - Avoid contact between solutions and metal objects (tweezers...)