Staining solution reagent Mallory trichrome

for histologyfor cytology

Minimum number of tests that can be performed 100 Completion time 20 minutes Shelf life 2 years Storage conditions 15-25°C Additional equipment Not required Application The standard method for viewing connective tissue on histological sections; particularly indicated for highlighting collagen, reticulum, cartilage, bone and amyloid. Result Nuclei, neurofibrils, myoglia, cartilage and bone tissue red Collagen fibrils blue Myelin golden yellow Elastic fibers pale pink – yellow or colorless Erythrocytes yellow Product for the preparation of cyto-histological samples for optical microscopy. Standard procedure for connective tissue; it shows collagen, reticulum, cartilage, bone, amyloid. PRINCIPLE In this method, three different dyes are used: carbol fuchsin for nuclear staining, orange G for cytoplasm and aniline blue for a selective collagen staining. Selectivity in this procedure is due to different degrees of affinity between dyes and tissue macromolecules. A central role is played by phosphomolibdic acid, which acts as a bound between tissue structures (collagen fibrils, cell membranes), and aniline blue (amphoteric dye). Orange G, which is the second component in Mallory’s polychrome solution, has no affinity to phosphomolibdic acid and is thus used to stain all remaining structures unbound to phosphotungstic acid. METHOD 1) Bring section to distilled water. 2) Put on the section 10 drops of reagent A: leave to act 10 minutes. 3) Wash in distilled water. 4) Put on the section 10 drops of reagent B: leave to act 2 minutes. 5) Wash quickly in tap water (2-3 seconds) and put on the section 10 drops of reagent C: leave to act 5 minutes.