Staining solution reagent Masson Fontana

for histologyfor cytology

Minimum number of tests that can be performed 100 Completion time 45 minutes + overnight Shelf life 1 year Storage conditions 2-8°C Additional equipment Not required Application The method of choice for viewing melanin pigment on sections of histological tissue. Result Melanin pigment brick red - black in the section under examination; absent in the control section (the presence of black precipitate on the control section indicates a false positive) Nuclei pink Product for the preparation of cyto-histological samples for optical microscopy. Recommended method to show melanin in histologic sections. PRINCIPLE Argentaffine reaction is based on the intrinsic capacity of some tissue components to reduce silver in an ammoniacal solution to metallic silver. To point out false positive results, this kit includes reagents for depigmentation of melanin (Mallory’s bleach). Depigmentation has to be effected on control section before silver impregnation. WARNING For good results, follow these rules: - Always use excellent and chlorine-free distilled or deionized water. - Use only perfectly clean glassware. - Avoid deposit of dust on sections. Never touch solutions with metallic objects (tweezers etc.) - Fixatives containing heavy metal salts (mercury chloride, potassium dichromate) should be avoided since deposits of such salts may cause false positive results. It is necessary to use distilled water for all the washings and perfectly clean glassware.