Staining solution reagent Masson Trichrome

for histologyfor cytology

Minimum number of tests that can be performed 100 Completion time 35 minutes Shelf life 2 years Storage conditions 15-25°C Additional equipment Not required Application The method of choice for connective tissue, particularly indicated for gametes, nuclei, neurofibrils, glia, collagen, keratin, intracellular fibrils and negative images of the Golgi apparatus. Result Nuclei and gametes black Cytoplasm, keratin, muscle fibers, acidophilic granules red Collagen, mucus, basophilic granules of the pituitary gland blue Delta cell granules of the pituitary gland blue Erythrocytes yellow Method 1) Bring the section to the distilled water. 2) Dispense 6 drops of reagent A onto the section and add 6 drops of reagent B: leave to act for 10 minutes. 3) Without washing, drain the slide and dispense 10 drops of solution C onto the section: leave to act for 4 minutes. 4) Wash quickly (3-4 seconds) in distilled water, leaving the section yellow in color, and dispense 10 drops of solution D onto the slide: leave to act for 4 minutes. 5) Wash in distilled water and dispense 10 drops of solution E onto the section: leave to act for 10 minutes. 6) Without washing, drain the slide and dispense 10 drops of solution F onto it: leave to act for 5 minutes. 7) Wash in distilled water and dehydrate rapidly by means of the ascending series of alcohols, pausing for 1 minute in the last absolute: xylene and balsam