Staining solution reagent Masson trichrome Goldner

for histologyfor cytology

Minimum number of tests that can be performed 100 Completion time 35 minutes Shelf life 2 years Storage conditions 15-25°C Additional equipment Not required Application The method of choice for connective tissue, indicated for highlighting gametes, nuclei, neurofibrils, glia, collagen, keratin, intracellular fibrils and negative images of the Golgi apparatus. Particularly indicated for black and white micro-photography. Result Nuclei and gametes black Cytoplasm, keratin, muscle fibers, acidophilic granules red Collagen, mucus, basophilic granules of the pituitary gland green Delta cell granules of the pituitary gland green Erythrocytes yellow Product for the preparation of cyto-histological samples for optical microscopy. Recommended method for connective tissue. It demonstrates gametes, nuclei, neurofibrils, neuroglia, collagen, keratin, intracellular fibrils, and negative image of Golgi apparatus. It is very suitable for black and white microphotographs. PRINCIPLE Four different stains are used: Weigert’s iron hematoxylin for nuclei, picric acid for erythrocytes, and a mixture of acid dyes (acid fuchsin-”ponceau de xylidine”) for cytoplasm and light green for collagen. METHOD 1) Bring section to distilled water. 2) Put on the section 6 drops of reagent A and add 6 drops of reagent B: leave to act 10 minutes. 3) Without washing, drain the slide and put on the section 10 drops of reagent C: leave to act 4 minutes. 4) Wash quickly (3-4 seconds) in distilled water and put on the section 10 drops of reagent D, leave to act 4 minutes. 5) Wash in distilled water and put on the section 10 drops of reagent E: leave to act 10 minutes.