Staining solution reagent Mucicarmine

for histologyfor cytology

Minimum number of tests that can be performed 100 Completion time 50 minutes Shelf life 1 year Storage conditions 15-25°C Additional equipment Graduated pipette Application Method indicated for highlighting acid mucopolysaccharides of epithelial nature (mucins) on histological sections. The use of Mucicarmine is of relative specificity, in fact mucins deriving from fibroblasts are generally weakly highlighted. Result Mucins from dark pink to red Nuclei blue – violet Other components orange Product for the préparation of cyto-histological samples for optical microscopy. To show acid mucopolysaccharides of épithélial origin (mucins) in histologie sections. Mucicarmine is comparatively spécifie; in fact, mucins derived from fibroblasts stain poorly. PRINCIPLE This is an empirical method and its histochemical principle is unknown. In Burck's opinion, the glucidic part of mucines reacts with carminé. METHOD 1) Bring section to distilled water. 2) Put on the section 10 drops of reagent A: leave to act for 5 minutes. 3) Blue in running tap water for 5 minutes. 4) Prepare the incubation box and introduce the slide 5) Put on the section 10 drops of reagent B and leave to act 30 minutes. 6) Wash in distilled water. 7) Put on the section 10 drops of reagent C: leave to act for 1 minute. 8) Wash in distilled water. 9) Dehydrate rapidly through ascending alcohols, stopping in the last absolute ethanol, clear in xylene and mount.