Staining solution reagent Oil red 0

for histologyfor cytology

Minimum number of tests that can be performed 100 Completion time 25 minutes Shelf life 2 years Storage conditions 15-25°C Additional equipment Glass histology jar with lid Application Method indicated for highlighting lipids on cryostat sections of tissue having a thickness of 5 μm. Fixation: you are advised to use saline formalin or Baker fixative in order to make the phospholipids less soluble. Result Fatty acids bright red Nuclei blue Product for the préparation of cyto-histological samples for optical microscopy. Recommended method for démonstration of lipids on 5 pm frozen sections. Fixation: it is recommended the use of Saline formalin or Baker1 s fixative in order to maintain the phospholipids. PRINCIPLE Oil red O solution stains selectively cellular lipids while hematoxylin solution stains nuclei. METHOD 1) Bring the sections to the distilled water. 2) Put the reagent A in a Coplin Jar and immerse the slide for 20 minutes. 3) Wash quickly in tap water. 4) Drain the slide and put on the section 10 drops of reagent B: leave to act 30 seconds. 5) Wash in running tap water for 3 minutes. 6) Drain and mount in aqueous medium.