Staining solution reagent P.A.S. 04-130802

for histologyfor cytology

Minimum number of tests that can be performed 100 Completion time 50 minutes Shelf life 1 year Storage conditions 2-8°C Additional equipment Not required Application For highlighting normal or pathological tissue components, distinguished by adjacent glycol or amino hydroxyl groups on histological sections and on blood smears and cytology smears. Result PAS-positive substances magenta red Nuclei blue Product for the préparation of cyto-histological samples for optical microscopy. To demonstrate normal and pathologie tissue components characterized by adjacent glycolic or aminohydroxylic groups for histological sections and for Haematology and cytology. PRINCIPLE Periodic acid oxidizes selectively the following groups: 1,2 glycolic; primary aminic (l-hydroxy-2-aminic); secondary aminic (1-hydroxy-2-alkylaminic); l-hydroxy-2-ketonic. Some methoxyl dérivatives and alpha-ketones are oxidized as well, but they are not converted to aldéhydes. During oxidation process the links between carbon atoms in 1,2 position break and consequently aldehydic groups are formed. In the following reaction, sulphurous fuchsin in Schiff reagent changes these two contiguous aldehydic groups into an insoluble stained compound similar to basic fuchsin. Three conditions are necessary for these reactions to take place: METHOD FOR HEMATOLOGY AND CYTOLOGY 1) Bring section to distilled water. 2) Put on the smear 10 drops of reagent A: leave to act 10 minutes. 3) Wash in distilled water. 4) Put on the smear 10 drops of reagent B: leave to act 15 minutes. 5) Wash in distilled water. 6) Put on the smear 10 drops of reagent C: leave to act 2 minutes.