Minimum number of tests that can be performed 100
Completion time 60 minutes
Shelf life 1 year
Storage conditions 2-8°C
Additional equipment Not required
Application
Digestion on a histological section with an amylase solution followed by PAS reaction
is indicated when you want to remove the glycogen so as to observe only the neutral
epithelial mucins.
PAS/amylase reaction is the method of choice for evaluating the presence of glycogen in
liver tissue on sections fixed in formalin and embedded in paraffin, and in muscle tissue
on cryostat sections.
In both cases, the examination of adjacent sections, one of which has been treated with
amylase, allows qualitative evaluation of the presence of glycogen.
Result
PAS-positive substances magenta red
Nuclei blue