Minimum number of tests that can be performed 72
Completion time 35 minutes
Shelf life 2 years
Storage conditions 15-25°C
Additional equipment 50 ml vertical histology jar, graduated cylinder and glass
rod
Application
Method indicated for simultaneous highlighting of reactive ferric iron, collagen and
connective tissue on tissue sections.
Result
Reactive ferric iron blue
Collagen purple red
Cytoplasm, muscle, stratum corneum of the epithelium, glia and
erythrocytes
yellow
Product for the préparation of cyto-histological samples for optical microscopy. To show ferrie iron, collagen and connective tissue in sections.
PRINCIPLE
Potassium ferrocyanide reacts with ferrie ions of hemosiderin in acid environment to form a coloured sait: Prussian blue. Reaction takes place in ionic form as follows: 4 Fe~~ 3K4Fe(CN)6 = Fe4(Fe(CN)6h+ 12 K*
A counterstain with Van Gieson Picrofuchsine differentiates collagen from connective tissue.