Staining solution reagent 04-021822

for histologyfor cytology

Minimum number of tests that can be performed 100 Completion time 40 minutes Shelf life 2 years Storage conditions 15-25°C Additional equipment Not required Application Trichrome stain recommended for connective sections. Result Nuclei: dark brown Collagen fibres: dark blue Ground substance of cartilage, bone, mucus, basophil granules of hypophysis and amyloid: shades of blue Neuroglia, axis cylinders and fibrin: red Acidophil granules of hypophysis: orange Myelin and erythrocytes: Yellow Elastic fibres: pale pink to yellow Product for the preparation of cyto-histological samples for optical microscopy. Trichrome stain recommended for connective sections. PRINCIPLE In this method, some components of connective tissue are selectively stained according to the different degree of affinity between dyes and tissue macromolecules. In particular, connective tissue is markedly acidophil because of the presence of many basic groups; therefore, it shows a strong affinity to acid dyes (picric acid and orange G) and a medium affinity to weak basic amphoteric dyes (acid fuchsin and ponceau xylidine). A central role is played by phosphomolybdic acid that acts as a bridge between tissue structures it attaches to (collagen fibrils, cell membranes) and aniline blue, a markedly basic amphoteric dye.