Stain reagent Picrosirius Red

staining solutioncollagenfor cytology

Minimum number of tests that can be performed 100 Completion time 60 minutes Shelf life 2 years Storage conditions 15-25°C Additional equipment Not required Application Method indicated for highlighting collagen fibers and bile pigments on tissue sections fixed in formalin and embedded in paraffin. Result Bilirubin green Collagen fibers red Nuclei blue Erythrocytes red Product for the preparation of cyto-histological samples for optical microscopy. For demonstration of collagen fibres and bilirubin in tissue sections. PRINCIPLE Sirius red picrate solution performs a double function: - to stain collagen by the binding between Sirius red and some proteins containing basic amino acids - to stain bilirubin by conjugation by picric acid Method 1) Bring the section to the distilled water. 2) Dispense 10 drops of reagent A onto the section: leave to act for 50 minutes. 3) Wash briefly in distilled water. 4) Dispense 10 drops of reagent B onto the section: leave to act for 2 minutes. Repeat twice. 5) Wash briefly in distilled water and drain the slide. 6) Dispense 10 drops of reagent C onto the section: leave to act for 3 minutes. 7) Leave to develop in tap water: 3 minutes. 8) Wash in distilled water and dehydrate rapidly by means of the ascending series of alcohols, leaving for 1 minute in the last absolute: xylene and balsam.